杨艳坤

职称：副教授

学历/学位：研究生/博士

电话：18651036633

电子邮箱：yangyankun@jiangnan.edu.cn

江苏省“六大人才高峰”入选者。从事发酵工程、微生物学、分子生物学、合成生物学的研究，主要承担分子生物学、生物化学、高级分子生物学课程的讲授工作。发表SCI研究论文60多篇，参编专著3部；申请发明专利20多项；主持国家重点研发计划、国家自然科学基金等国家级和省部级科研项目9项；主持企业横向项目10项；获得商业联合会全国科技进步2等奖1项；指导学生获得国家级和省级竞赛奖4项。

1997.09-2001.07，东北师范大学，生物技术专业，理学学士

2001.09-2006.07  中国科学院武汉病毒研究所，微生物学专业，理学博士

2006.07-2013.05  郑州大学，生物工程系，讲师

2013.05-今    3044am永利集团3044noc，3044am永利集团3044noc/粮食发酵与食品生物制造国家工程研究中心，副教授

2004.02-2005.06  英国卡迪夫大学，访问学者

2018.12-2019.12  美国伦斯勒理工学院，访问学者

一、论文（论著）发表情况

部分代表性论文如下：

1. Liu, X.X., et al., Enhanced molecular stability of ApxII antigen during secretion in Corynebacterium glutamicum by rational design. Journal of Biotechnology, 2024. 394: p. 73-84.

2. Zhan, C., et al., Reprogramming methanol utilization pathways to convert Saccharomyces cerevisiae to a synthetic methylotroph. Nature Catalysis, 2023. 6(5): p. 435-450.

3. Sun, M., et al., High-throughput process development from gene cloning to protein production. Microb Cell Fact, 2023. 22(1): p. 182.

4. Lin, P., et al., Direct Utilization of Peroxisomal Acetyl-CoA for the Synthesis of Polyketide Compounds in Saccharomyces cerevisiae. ACS Synth Biol, 2023. 12(6): p. 1599-1607.

5. Li, Y., et al., Plasmid Copy Number Engineering Accelerates Fungal Polyketide Discovery upon Unnatural Polyketide Biosynthesis. ACS Synth Biol, 2023. 12(8): p. 2226-2235.

6. Li, S., et al., Development and characterization of anti-IL-5 monoclonal antibody Fab fragment for blocking IL-5/IL-5Rα binding. International Immunopharmacology, 2023. 124: p. 111032.

7. Li, S., et al., Rapid Screen of IL-5/IL-5Rα Blocking Antibodies in the HEK293-IL-5Rα-CSF2RB Transfected Cell Line. Biotechnology and Bioprocess Engineering, 2023. 28(4): p. 612-622.

8. Hu, L., et al., Creating NADP+-Specific Formate Dehydrogenases from Komagataella phaffii by Enzymatic Engineering. ChemBioChem, 2023. 24(24): p. e202300587.

9. Fordjour, E., et al., Engineering Escherichia coli BL21 (DE3) for high-yield production of germacrene A, a precursor of β-elemene via combinatorial metabolic engineering strategies. Biotechnol Bioeng, 2023. 120(10): p. 3039-3056.

10. Yu, X., et al., Development of a novel platform for recombinant protein production in Corynebacterium glutamicum on ethanol. Synthetic and Systems Biotechnology, 2022. 7(2): p. 765-774.

11. Li, Y., et al., Recent advances in high-throughput metabolic engineering: Generation of oligonucleotide-mediated genetic libraries. Biotechnology Advances, 2022. 59: p. 107970.

12. Han, F., et al., Advances in the Study of Inhaled Formulations for the Treatment of Pulmonary Arterial Hypertension. Applied Bionics and Biomechanics, 2022. 2022: p. 6495645.

13. Zhan, C., et al., Strategies and challenges with the microbial conversion of methanol to high-value chemicals. Biotechnology and Bioengineering, 2021. 118(10): p. 3655-3668.

14. Wang, R., et al., MAPK/HOG signaling pathway induced stress-responsive damage repair is a mechanism for Pichia pastoris to survive from hyperosmotic stress. Journal of Chemical Technology & Biotechnology, 2021. 96(2): p. 412-422.

15. Han, S., et al., The Development of Novel N-Terminal Pro-Peptide of Type I Chemiluminescence Assay. Clin Lab, 2021. 67(9).

16. Han, F., et al., Interleukin-6 promotes ferroptosis in bronchial epithelial cells by inducing reactive oxygen species-dependent lipid peroxidation and disrupting iron homeostasis. Bioengineered, 2021. 12(1): p. 5279-5288.

17. Yang, Y., et al., Characterization and application of a putative transcription factor (SUT2) in Pichia pastoris. Molecular Genetics and Genomics, 2020. 295(5): p. 1295-1304.

18. Yang, Y., et al., Glycerol transporter 1 (Gt1) and zinc-regulated transporter 1 (Zrt1) function in different modes for zinc homeostasis in Komagataella phaffii (Pichia pastoris). Biotechnology Letters, 2020. 42(11): p. 2413-2423.

19. Yang, Y., et al., High efficiency CRISPR/Cas9 genome editing system with an eliminable episomal sgRNA plasmid in Pichia pastoris. Enzyme and Microbial Technology, 2020. 138: p. 109556.

20. Nie, J., et al., Production Process Development of Pseudorabies Virus Vaccine by Using a Novel Scale-Down Model of a Fixed-Bed Bioreactor. Journal of Pharmaceutical Sciences, 2020. 109(2): p. 959-965.

21. Nie, J., et al., Pseudorabies virus production using a serum-free medium in fixed-bed bioreactors with low cell inoculum density. Biotechnology Letters, 2020. 42(12): p. 2551-2560.

22. Nie, J., et al., Rapid process development of serum-free pseudorabies virus production with the Quality by Design approach. Cytotechnology, 2020. 72(2): p. 283-293.

23. Liu, X., et al., Identification, repair and characterization of a benzyl alcohol-inducible promoter for recombinant proteins overexpression in Corynebacterium glutamicum. Enzyme and Microbial Technology, 2020. 141: p. 109651.

24. Hou, C., et al., Targeted editing of transcriptional activator MXR1 on the Pichia pastoris genome using CRISPR/Cas9 technology. Yeast, 2020. 37(4): p. 305-312.

25. Han, S., et al., A Chemiluminescent Immunoassay for Osteocalcin in Human Serum and a Solution to the “Hook Effect”. Journal of Analytical Methods in Chemistry, 2020. 2020: p. 8891437.

26. Li, X.-R., et al., A scale-down model of 4000-L cell culture process for inactivated foot-and-mouth disease vaccine production. Vaccine, 2019. 37(43): p. 6380-6389.

27. Li, X., et al., Quality by Design-Driven Process Development of Severe Fever With Thrombocytopenia Syndrome Vaccine. Journal of Pharmaceutical Sciences, 2019. 108(12): p. 3785-3791.

28. Li, X., et al., Quality by Design-Driven Process Development of Cell Culture in Bioreactor for the Production of Foot-And-Mouth Veterinary Vaccine. Journal of Pharmaceutical Sciences, 2019. 108(7): p. 2288-2295.

29. Yang, Y., et al., Correlation Between Protein Primary Structure and Soluble Expression Level of HSA dAb in Escherichia coli. Food Technology and Biotechnology, 2018. 56(1): p. 101-109.

30. Li, X., et al., Transcriptional analysis of impacts of glycerol transporter 1 on methanol and glycerol metabolism in Pichia pastoris. FEMS Yeast Research, 2018. 18(1): p. fox081.

31. Zhan, C., et al., Transcription factor Mxr1 promotes the expression of Aox1 by repressing glycerol transporter 1 in Pichia pastoris. FEMS Yeast Research, 2017. 17(4): p. fox015.

32. Zhan, C., et al., The Pichia pastoris transmembrane protein GT1 is a glycerol transporter and relieves the repression of glycerol on AOX1 expression. FEMS Yeast Research, 2016. 16(4): p. fow033.

33. Chen, A., et al., Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli. Microbial Cell Factories, 2016. 15(1): p. 1-10.

二、专利情况

1.杨艳坤等. 表达外源蛋白的毕赤酵母、其构建方法及其诱导表达方法, CN201610623309.5.

2.杨艳坤等. 抗人血清白蛋白单链抗体及其碳端连接多肽药物的方法, CN201210125323.4.

3.杨艳坤等. 一种蛋白质, CN201510084726.2.

4.杨艳坤等. 一种巴斯德毕赤酵母不同碳源下的内参基因及其引物和应用, CN114574617A

5.杨艳坤等. 一种巴斯德毕赤酵母组成型启动子及其改造方法和应用, CN114574490A

三、承担教学科研项目情况

1.国家自然科学基金委员会，面上项目，32370054，解析与重构毕赤酵母还原性甘氨酸途径实现甲醇和CO2的协同固定，2024-01至2027-12，主持。

2.军委科技委，面向军用蛋白XXX装备与技术，2022-10-1至2024-09-30，参与。

3.国家重点研发计划子课题（课题号2021YFC2100203），所属专项：绿色生物制造；所属项目：工业酶通用高效表达系统构建；课题名称：工业酶酵母高效表达系统全局设计优化与创建；2021-07至2024-06，子课题负责人。

4.国家自然科学基金委员会，面上项目，31570034，巴斯德毕赤酵母中甘油通过GT1和MXR1调控甲醇代谢的分子机理研究，2016-01至2019-12，主持。

5.教育部，中央高校基本科研业务费专项资金资助项目，JUSRP51401A，基于微反应器的HTP细胞筛选技术及ScFv谷氨酸棒杆菌表达纯化系统的建立，2014-01至2016-12，主持。

6.国家自然科学基金委员会，青年基金，31001031，紫花苜蓿可调控的花发育抑制系统的构建及其在苜蓿种质创新中的应用，2010-01至2012-12，主持。

7.主持江苏省产学研项目1项（项目号BY2015019-17），计划类别：政策引导类计划（产学研合作）--前瞻性联合研究项目；维生素B12、维生素D、叶酸免疫检测产品的研发，2015-07至2017-06，主持。

8.3044am永利集团3044noc研究生教育教学改革研究与实践课题，主持。

9.优化课程教学体系建设服务于科研的高级分子生物学课程，主持。

10.卓越课程建设项目：分子生物学，主持。

11.研究生在线课程建设项目：高级分子生物学，主持。

四、获奖情况（含指导学生获奖）

1.全国商业联合会科技进步奖二等奖，1/12。

2.中国国际大学生创新大赛（2024）省赛二等奖，第1指导老师

3.第三届全国大学生生命科学创新创业大赛国家一等奖，第1指导老师

4.“创青春”全国大学生创业大赛国家铜奖，第1指导老师

5.第四届中国“互联网+”大学生创新创业大赛省二等奖，第1指导老师 

以上资料更新时间截止：2024年9月